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A linearized DNA template was transcribed using T7 RNA polymerase according to standard in vitro transcription conditions
The best value available anywhere for simple cloning and plasmid propagation
2 mM dithiothreitol
and prepare the RNA for cDNA synthesis
YES: without IPTG induction
E. cloni-10G ELITE Electrocompetent Cells (SOLOs) size:12 rxns A linearized DNA template wasE. cloni 10G and 10GF' Electrocompetent Cells Direct replacements for standard cloning strains (e. g., DH5, DH10B, JM109, TOP10, XL1 Blue, etc.) Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue white screening capable. Available in a range of high transformation efficiencies (5 109 to 4 1010 cfu g). Convenient packaging options. Lucigens E. cloni competent cells share the most useful genetic elements
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